Vagus Nerve Stimulation (VNS) Original Article| Volume 8, ISSUE 3, P624-636, May 2015

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Non-invasive Access to the Vagus Nerve Central Projections via Electrical Stimulation of the External Ear: fMRI Evidence in Humans

  • Eleni Frangos
    Corresponding author. Department of Psychology, Rutgers University, Room 304, 101 Warren Street, Newark, NJ 07102, USA. Tel.: +1 201 233 7982.
    Department of Psychology, Rutgers University, 101 Warren St, Newark, NJ 07102, USA
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  • Jens Ellrich
    Cerbomed GmbH, Henkestrasse 91, 91052 Erlangen, Germany

    Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 7D2, DK-9220 Aalborg, Denmark

    Institute of Physiology and Pathophysiology, Friedrich-Alexander-University Erlangen-Nuremberg, Universitaetsstrasse 17, D-91054 Erlangen, Germany
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  • Barry R. Komisaruk
    Department of Psychology, Rutgers University, 101 Warren St, Newark, NJ 07102, USA
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Published:December 05, 2014DOI:


      • Electrical stimulation of the cymba conchae activates the NTS in humans.
      • Activations and deactivations of vagal projections were found throughout the brain.
      • The pattern of activity can account for the beneficial effects of t-VNS and VNS.



      Tract-tracing studies in cats and rats demonstrated that the auricular branch of the vagus nerve (ABVN) projects to the nucleus tractus solitarii (NTS); it has remained unclear as to whether or not the ABVN projects to the NTS in humans.


      To ascertain whether non-invasive electrical stimulation of the cymba conchae, a region of the external ear exclusively innervated by the ABVN, activates the NTS and the “classical” central vagal projections in humans.


      Twelve healthy adults underwent two fMRI scans in the same session. Electrical stimulation (continuous 0.25ms pulses, 25Hz) was applied to the earlobe (control, scan #1) and left cymba conchae (scan #2). Statistical analyses were performed with FSL. Two region-of-interest analyses were performed to test the effects of cymba conchae stimulation (compared to baseline and control, earlobe, stimulation) on the central vagal projections (corrected; brainstem P < 0.01, forebrain P < 0.05), followed by a whole-brain analysis (corrected, P < 0.05).


      Cymba conchae stimulation, compared to earlobe (control) stimulation, produced significant activation of the “classical” central vagal projections, e.g., widespread activity in the ipsilateral NTS, bilateral spinal trigeminal nucleus, dorsal raphe, locus coeruleus, and contralateral parabrachial area, amygdala, and nucleus accumbens. Bilateral activation of the paracentral lobule was also observed. Deactivations were observed bilaterally in the hippocampus and hypothalamus.


      These findings provide evidence in humans that the central projections of the ABVN are consistent with the “classical” central vagal projections and can be accessed non-invasively via the external ear.


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